Peripheral blood mononuclear cells (PBMCs) from a CMV seropositive donor, were obtained through a collaboration. Cells were stained with a panel of 12 dCODE™ Dextramer® reagents (Immudex) and 17 TotalSeq™-C antibodies (BioLegend). See details in the Application Note “Redefining Cellular Phenotyping: Comprehensive Characterization and Resolution of the Antigen-Specific T Cell Response”.
The gene expression and cell surface protein libraries from the sorted and unsorted samples were aggregated using Cell Ranger aggr.
- 7,346 total cells detected
- 1,438 median genes per cell and 4,524 median UMI counts per cell detected
- ~53,000 read pairs per cell pre-normalization; ~49,000 reads per cell post-normalization