<SupportCallout>Compatible with GEM-X Flex v2 (GEM-X Flex Apex)</SupportCallout><br />

Outlines how to perform fixation on single cell and nuclei suspensions for GEM-X Flex Gene Expression.


Outlines how to perform fixation on single cell and nuclei suspensions for GEM-X Flex Gene Expression.

这种基于探针的分析利用GEM-X技术捕获18,000多个编码基因，可根据项目需求进行定制。

Flex基因表达

Chromium X系列单细胞系统

Chromium

Advanced system for multiomic analysis of hundreds to millions of cells.

Flex Gene Expression

Gene Expression

Epigenomic Profiling

Immune Profiling

Protein co-detection

Fixation of Cells & Nuclei for GEM-X Flex Gene Expression

Prepare single cell/nuclei suspension from fresh samples for fixation or from archived samples by following our Demonstrated Protocols.

Sample Prep

Flex Gene Expression enables single cell gene expression on precious samples that were previously inaccessible due to logistical challenges in sample handling. Fixation at the point of sample collection preserves fragile biology and greatly streamlines workflows to provide critical insights on more samples.

Reveal gene expression and protein expression from the same fixed cell.

Flex with Protein Labeling

Protein

It is critical to choose the User Guide / Protocol that corresponds to your Reagent Kit version. Find the right User Guide via the button below.

Additional Applications

Measure single cell gene and cell surface protein expression simultaneously.

Cell Surface Protein

Find resources from protocols to free-to-use analysis tools.

Plan ahead by referring to the list of equipment, kits, and reagents needed for Flex Gene Expression.

Experimental Design

Experimental Design & Planning

Find a list of probes targeting protein-coding genes in human and mouse transcriptomes for the Flex Gene Expression assay.

Probe Sets

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Library Construction

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Sequence your single cell or nuclei library as per our provided recommendations.

Sequencing

Analyze your genome and exome data using our pipelines and visualization tools.

Software Analysis

Workflow Documentation

Overview

User Guides

Datasets

Videos

What is Flex Gene Expression?

Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

GEM-X: How to assemble, load and generate GEMs

Chromium Next GEM Tissue Fixation and Dissociation

Chromium Next GEM Single Cell Isolation from FFPE Sections

Find answers to common technical questions, from sample prep through data analysis, about the Flex Gene Expression workflow.

Fixation of Cells & Nuclei for GEM-X Flex Gene Expression

Demonstrated Protocol, CG000782

CG000782_DemonstratedProtocol_Cell_NucleiFixation_GEM-X_Flex_GeneExpression_RevD.pdf