Visium HD Spatial Gene Expression User Guide 2.0 is compatible with 6.5mm and 11mm slide workflow. If the original protocol is required for use, consult Visium HD Spatial Gene Expression User Guide (CG000685)
This assay requires CytAssist instrument Firmware v2.0.0 or higher. Refer to the Visium CytAssist with Readiness Test User Guide (CG000542) for details.
Best Practice video series
Key Techniques for Visium HD Slide Preparation & Use
Best Practice video series
Key Techniques for Tissue Slide Handling & Use
This document outlines the protocol for generating Visium HD Spatial Gene Expression libraries from FFPE, Fresh Frozen, and Fixed Frozen tissue sections. Key companion documents for use with this document:
- Visium HD Spatial Gene Expression Protocol Planner (CG000698)
- Tissue Slide Alignment Instruction, Quick Reference Cards (CG000548)
- Visium Cassette S3 for Assembly and Disassembly Quick Reference Cards (CG000730)
For use with:
- Visium HD, Human Transcriptome, 6.5 mm, PN-1000675 (4 rxns) / PN-1000673 (16 rxns)
- Visium HD, Mouse Transcriptome, 6.5 mm, PN -1000676 (4 rxns) / PN-1000674 (16 rxns)
- Visium HD, Human Transcriptome, 11 mm, PN-1001037 (2 rxns) / PN-1001039 (8 rxns)
- Visium HD, Mouse Transcriptome, 11 mm, PN -1001038 (2 rxns) / PN-1001040 (8 rxns)
- Visium CytAssist Reagent Accessory Kit, PN-1000499
- Dual Index Kit TS Set A, 96 rxns, PN-1000251