Visium HD Fixed Frozen Tissue Preparation Handbook 2.0 is compatible with 6.5mm and 11mm slide workflow. If the original protocol is required for use, consult Visium HD Fixed Frozen Tissue Preparation Handbook (CG000764)
When working with Fixed Frozen tissues, generating sections with uniform slide adhesion can be challenging due to their sample composition. Please refer to the Visium HD Spatial Gene Expression Protocol Planner (CG000698) Tested Slides section for slide part number recommendations and additional considerations.
This document provides guidance on preparation of Fixed Frozen tissue blocks and generating tissue slides. Demonstrated protocols for performing tissue staining (H&E and IF) are included.
Key companion documents for use with this document:
- Visium HD Spatial Gene Expression Protocol Planner (CG000698)
- Tissue Slide Alignment Instruction, Quick Reference Cards (CG000548)
- Visium S3 for Tissue Slide Cassette Assembly Quick Reference Cards (CG000730)
For use with:
- Visium HD, Human Transcriptome, 6.5 mm, PN-1000675 (4 rxns) / PN-1000673 (16 rxns)
- Visium HD, Mouse Transcriptome, 6.5 mm, PN -1000676 (4 rxns) / PN-1000674 (16 rxns)
- Visium HD, Human Transcriptome, 11 mm, PN-1001037 (2 rxns) / PN-1001039 (8 rxns)
- Visium HD, Mouse Transcriptome, 11 mm, PN -1001038 (2 rxns) / PN-1001040 (8 rxns)
- Visium CytAssist Reagent Accessory Kit, PN-1000499
- Dual Index Kit TS Set A, 96 rxns, PN-1000251