5k Mouse Kidney, Chromium GEM-X Epi Multiome with Automated Cell Annotation

Snap-frozen normal mouse kidney tissue was obtained by 10x Genomics from AcePix Biosciences. Nuclei were isolated following the Nuclei Isolation from Complex Tissues for Single Cell Multiome ATAC + Gene Expression Sequencing Demonstrated Protocol (CG000375, Rev D). Paired ATAC and Gene Expression libraries were generated as described in the Chromium GEM-X Epi Multiome User Guide (CG001689, Rev B) and sequenced on an Illumina NovaSeq 6000 (ATAC) and Illumina NovaSeqX+ (GEX) with approximately 40,000 ATAC read pairs per cell and 60,000 GEX read pairs per cell.

Gene Expression library, paired-end, dual indexed:

• 28 cycles Read 1
• 10 cycles i7
• 10 cycles i5
• 90 cycles Read 2

ATAC library, paired-end, single index:

• 50 cycles Read 1
• 8 cycles i7
• 24 cycles i5
• 49 cycles Read 2

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.