Learn more about a change in the recommended chip assembly and loading technique

What do I need to know?

• 10x Genomics is committed to providing customers the best experience to support their research needs.
• We have observed that, on occasion, the gasket may misalign to our Chromium chips when assembling per our current workflow guidance for the assays listed below.
• The misalignment most often results in a pressure error occurring early in the run on the Chromium X/iX or Chromium Controller. If this occurs, it can usually be resolved by re-opening and closing the secondary holder lid to allow the gasket to re-align with the wells on the chip.
• To reduce the risk of gasket misalignment, we recommend assembling the gasket onto the secondary holder before inserting the chip and loading the reagents.
• This change of workflow will not have an impact on the experiment or data.
• Link to Customer Notification Letter here

Please reference the following affected assays:

• Chromium Single Cell Gene Expression (LT and Standard Throughput)
• Chromium Single Cell Immune Profiling (Standard Throughput)
• Chromium Single Cell Multiome ATAC + Gene Expression
• Chromium Single Cell ATAC
• Chromium Fixed RNA Profiling

List of Chromium chip impacted

New How-To Video Available

Please click HERE to view a video demonstration of the updated technique and step-by-step guidance.