For imaging hardware, general image acquisition and analysis guidelines, and example images.

Imaging

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium Spatial Gene Expression for FFPE protocols.

Experimental Design

For proper FFPE tissue sectioning and placement.

Deparaffinize, stain, image, and decrosslink tissue.

Find a list of probes targeting protein-coding genes in human or mouse transcriptome for the Visium for FFPE assay.

Construct a sequencing-ready Visium spatial library from your FFPE tissue sections.

Sequence your Visium spatial library as per our provided recommendations.

Analyze your Visium spatial data using our pipelines and visualization tools.