HD 3’ Spatial Gene Expression

What is HD 3’ Spatial Gene Expression?
Single cell-scale resolution, unbiased, whole transcriptome, poly-A based gene expression assay compatible with Fresh Frozen tissue sections.
Documentation
Find resources, from protocols to free-to-use analysis tools.
Plan ahead by referring to the list of equipment, kits, and reagents needed for Visium HD 3’ Spatial Gene Expression protocols.
Best practices for preparing and handling Fresh Frozen tissue samples and generating tissue slides. Protocol outlines fixation, staining, and imaging of tissue sections.
For imaging hardware, general image acquisition and analysis guidelines, and example images.
Construct a sequencing-ready Visium HD 3’ spatial library from Fresh Frozen tissue sections.
Refer to instrument-specific documents for seamless instrument operation.
Sequence your Visium HD 3’ spatial library as per our provided recommendations.
Analyze your Visium spatial data using our pipelines and visualization tools.
Datasets
Access free, publicly available data from a number of different sample types generated with Visium HD 3’ Spatial Gene Expression

Q&A
Find answers to common technical questions, from sample prep through data analysis, about Visium spatial workflows.