Plan ahead by referring to the list of equipment, kits, and reagents needed for Visium HD 3’ Spatial Gene Expression protocols.

Experimental Design

Experimental Planning and Design

Best practices for preparing and handling Fresh Frozen tissue samples and generating tissue slides. Protocol outlines fixation, staining, and imaging of tissue sections.

Tissue Prep & Staining

For imaging hardware, general image acquisition and analysis guidelines, and example images.

Imaging

Construct a sequencing-ready Visium HD 3’ spatial library from Fresh Frozen tissue sections.

Library Construction

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Sequence your Visium HD 3’ spatial library as per our provided recommendations.

Sequencing

Analyze your Visium spatial data using our pipelines and visualization tools.

User Guides