GEM-X technology-powered assays with whole transcriptome coverage to gather broad information from diverse species

Universal 3'

Chromium X Series

Chromium

Advanced system for multiomic analysis of hundreds to millions of cells.

Gene Expression

Epigenomic Profiling

Immune Profiling

Protein co-detection

3' gene expression profiling at scale with single cell resolution.

GEM-X technology-powered assays with whole transcriptome coverage to analyze immune diversity 

Universal 5'

5' gene expression alongside V(D)J repertoire profiling and antigen specificity of T and B cells.

Single cell profiling of open chromatin and 3' gene expression from the same nucleus

Epi Multiome

Combined profiling of 3' gene expression and chromatin accessibility from the same nucleus.

Plate-based sample handling brings unprecedented scale to highly sensitive single cell profiling

Flex

Fixed RNA Profiling assay for comprehensive probe-based gene expression profiling with single cell resolution.

Visium CytAssist

Visium

Compact instrument designed to simplify the Visium workflow by facilitating the transfer of analytes from standard glass slides to Visium slides.

CytAssist Spatial Gene Expression

Probe-based assay allowing simultaneous analysis of molecular and imaging data from FFPE, Fresh Frozen, and Fixed Frozen tissue sections.

HD Spatial Gene Expression

Whole transcriptome probe-based gene expression assay at single cell scale, compatible with FFPE, Fresh Frozen, and Fixed Frozen tissue sections

Combine whole transcriptome spatial analysis with high-plex protein detection using NGS in the same tissue section. Gain a deeper, more holistic understanding of tissue organization, using Visium CytAssist to expand sample access and ease workflows.

Immunofluorescence protein co-detection

Visium CytAssist Gene and Protein Expression

Tissue profiling with transcriptomics and <em>protein co-detection</em>

CytAssist Spatial Gene and Protein Expression

Simultaneous analysis of RNA and protein expression of FFPE tissues, using probe-based chemistry and tagged antibodies.

Spatial Gene Expression for Fresh Frozen

Poly-A based assay allowing simultaneous analysis of molecular and imaging data from fresh frozen tissue sections.

RNase Inhibitor Safety Data Sheets

Prepare optimal single cell/nuclei suspension by following our Demonstrated Protocols.

Sample Prep

Chromium Single Cell Universal 3’ Gene Expression provides single cell transcriptome 3' gene expression alongside the detection of surface protein expression in tens of thousands of cells.

Measure single cell gene and cell surface protein expression simultaneously.

Single Cell Gene Expression with Cell Surface Protein Labeling

Cell Surface Protein

It is critical to choose the User Guide / Protocol that corresponds to your Reagent Kit version. Find the right User Guide via the button below.

Assess effects of CRISPR perturbations alongside single cell gene expression.

Single Cell Gene Expression with CRISPR Screening

CRISPR

Multiplex up to 12 samples per channel for increased sample throughput.

Single Cell Gene Expression with Cell Multiplexing technology

Cell Multiplexing

Profile 2,000–20,000 cells per channel of the Chromium Next GEM Chip M (2,000– 60,000 cells per channel with CellPlex).

Single Cell Gene Expression HT with Feature Barcode technology

High Throughput (HT)

Chromium Next GEM Single Cell 3' HT v3.1 - Dual Index

Seamless library construction capabilities on automated platforms.

3rd Party Automation

10x PAE Services are designed to help you automate your workflows with confidence.

Premium Automation Enablement (PAE)

Additional Applications

Find resources from protocols to free-to-use analysis tools.

Plan ahead by referring to the list of equipment, kits, and reagents needed for Single Cell Gene Expression.

Experimental Design

Experimental Design & Planning

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Library Construction

Use pre-designed or custom panels for targeted gene expression, followed by sequencing and data analysis.

Targeted

Targeted Gene Expression (Optional)

Sequence your single cell library as per our provided recommendations.

Sequencing

Analyze your single cell data using our pipelines, visualization, and Pipeline Runner tools.

Software Analysis

Workflow Documentation

User guides

How-to videos

FAQs

Universal 3’

Find answers to common technical questions, from sample prep through data analysis, about Chromium single cell workflows.

Knowledge base Q&A

Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

Datasets

Read key peer-reviewed research publications offering greater insights into biology, enabled by 10x Genomics products and technologies.

Publications

Other resources

Overview

User Guides

Videos

Universal 3' Gene Expression

GEM-X: How to assemble, load and generate GEMs

GEM-X OCM: How to assemble, load and generate multiplex GEMs

Chromium Next GEM Single Cell 3' v3.1 - Dual Index

Workflow Overview

Workflow overview and additional reagents for workflow. Before starting the experiment, consult these documents for protocol

Protocol Planner

General guidance on sample preparation best practices

Cell Preparation Handbook

General guidance on specific sample preparation protocols

Specific Sample Preparation Documents

Label cells with antibody-oligonucleotide conjugates for use with 10x Genomics Single Cells Protocols with Feature Barcode technology for Cell Surface Protein

Cell Surface Protein Labeling Demonstrated Protocol

Sample Preparation

Chromium GEM-X Single Cell 3' Reagent Kits v4

Construct 3' Gene Expression and Cell Surface Protein libraries

Chromium GEM-X Single Cell 3' Reagent Kits v4 with Feature Barcode technology for Cell Surface Protein

Provides instructions on constructing 3' Gene Expression libraries

GEM-X Universal 3' Gene Expression v4 4-plex Reagent Kits

Provides instructions on constructing 3' Gene Expression & cell surface protein libraries

GEM-X Universal 3' Gene Expression v4 4-plex Reagent Kits with Feature Barcode technology for Cell Surface Protein

Provides guidance and best practices for sequencing 10x Genomics libraries.

Sequencing Handbook

Analyze single cell gene expression data with a set of free, easy-to-use analysis pipelines.

Cell Ranger

Analyze 10x Genomics data quickly and securely in the cloud.

Pipeline Runner

Explore 10x Genomics data with our powerful visualization software. Compatible with single cell and spatial products.

RNase Inhibitor Safety Data Sheets

SDS

1000887_RNase_Inhibitor_40x_SDS_Ed1.pdf