Human Discovery Panel | 10x Genomics

MultiPro Human Discovery Panel

Same-cell protein capture for high-performance scRNA-seq

300+ proteins. One workflow. Full resolution.

Access unparalleled scale for protein discovery with 347 DNA-barcoded antibodies to profile up to 326 protein targets. This panel integrates seamlessly as part of our existing Flex scRNA-seq workflows and enables deeper insights from the same cells, in the same run.

With this comprehensive discovery panel, you can:

Detect cell surface and intracellular proteins, including cytokines, transcription factors, phosphoproteins, and more
Explore activation state and signaling pathways with the most diverse antibody-based single cell panel available
Generate robust, reproducible results with pre-titrated, single-use, lyophilized reagents

Validation data gallery

Download full dataset

Lewis x antigen involved in cell-cell adhesion, mediates phagocytosis and chemotaxis. UMAP shows resting bone marrow mononuclear cells

E-cadherin, mediates cell-cell adhesion in epithelial tissues. UMAP shows resting bone marrow mononuclear cells

Stem cell factor receptor, plays essential roles in hematopoiesis, spermatogenesis, and melanogenesis. UMAP shows resting bone marrow mononuclear cells

Protein kinase C and casein kinase substrate in neurons protein 1, involved in endocytosis and actin dynamics. UMAP shows human PBMCs stimulated with anti-CD3/CD28 for 4 hr

H2A histone family member X, involved in DNA repair and chromatin structure. UMAP shows human PBMCs stimulated with anti-CD3/CD28 for 4 hr

Transcription factor critical for hematopoietic differentiation and lymphatic system development. UMAP shows resting bone marrow mononuclear cells and in human PBMCs stimulated with LPS for 4 hr

Drebrin 1, involved in dendritic spine morphogenesis and synaptic plasticity. UMAP shows human PBMCs stimulated with LPS for 4 hr

Transcription factor critical for hematopoietic differentiation and lymphatic system development. UMAP shows resting bone marrow mononuclear cells and in human PBMCs stimulated with LPS for 4 hr

Stem cell factor receptor, plays essential roles in hematopoiesis, spermatogenesis, and melanogenesis. UMAP shows resting bone marrow mononuclear cells

N-cadherin, involved in cell-cell adhesion and signal transduction, crucial in the nervous system and heart. UMAP shows resting PBMCs.

Phosphorylated MAP kinase kinase 1 at Serine 298, part of the MAPK/ERK pathway, involved in cell growth signaling. UMAP shows resting PBMCs.

Transforming growth factor beta 1, regulates cell growth, proliferation, differentiation, and apoptosis. UMAP shows resting PBMCs.

Product information

Tested reactivity

Human

Host / isotype

Rabbit polyclonal IgG

Mouse IgG2a

IgG2b

Mouse IgG2b

Mouse IgG1

IgMk

Mouse IgG3

Mouse IgG2ak

Class

Monoclonal

Recombinant

Polyclonal

Type

DNA-barcoded antibody cocktail

Storage buffer

Lyophilized antibody cocktail

Storage conditions

Store at 2–8°C.

Targets

MultiPro Discovery Antibody Manifest

*This file contains macros that are used to make searching the list of antibodies easier. Make sure to enable macros when prompted. You may also need to “unblock” the file in the file’s properties box.

Download MultiPro Discovery Antibody Manifest G9000150

Documentation

GEM-X protocols

C10024 (Rev 0) MultiPro HDP w HashMax™-5 Staining Protocol for GEM-X Flex

C10027 (Rev 0) MultiPro Human Discovery Panel Protocol for GEM-X Flex

C10035 (Rev B) MultiPro Human Discovery Panel Staining Protocol for Tube-Based GEM-X Flex v2 Apex

SDS

Feature References

Human Discovery Panel Feature Reference

*The use of MultiPro Enhanced Blocking Reagent is highly recommended for improved signal-to-noise ratio. See protocol for more details.

Sequencing parameters

IMPORTANT: The sequencing parameters for MultiPro antibodies are distinct from those required for Flex Apex sequencing libraries. MultiPro sequencing parameters are also distinct from other commercially available oligonucleotide conjugated antibodies. Please be mindful of this when setting up your sequencing run. The required MultiPro sequencing parameters are:

If using Cell Ranger v8.0+

Read 1: 28 cycles
Index 1: 10 cycles
Index 2: 10 cycles
Read 2: 90 cycles

If using Cell Ranger v7.2

Read 1: 48 cycles
Index 1: 10 cycles
Index 2: 10 cycles
Read 2: 50 cycles