10x Instrument Requirements:
| Application | Chromium Controller | Chromium Xo | Chromium X | Chromium iX |
|---|---|---|---|---|
| Next GEM Epi Multiome (ATAC + Gene Expression) | Upgrade Required | |||
| GEM-X Epi Multiome | Upgrade Required |
- Installation of Firmware v2.0.0 or higher via network connection
- See the Chromium X Series: Network Connectivity Guidelines (CG000508) for details on getting connected and the "Firmware Update Procedure" section in the Chromium X Series (X/iX) User Guide with Readiness Test (CG000396) for firmware installation
Reagent Accessory Requirements:
- Chromium X Series Chip Holder (PN-3000598; previously named Chromium X/iX Chip Holder)*
- 10x Magnetic Separator B (PN-2001212; previously named 10x Magnetic Separator HT PN-2000431)*
*Included in the Chromium X Series Accessory Kit (PN-1000821) and GEM-X Transition Kit (PN-1000707)
| Chromium X/iX Chip Holder | 10x Magnetic Separator B |
|---|---|
 |  |
10x Genomics Reagent Kit Requirements:
- See the Chromium GEM-X Single Cell User Guides for details
For nuclei isolation Demonstrated Protocols, we strongly recommend using one of the following RNase inhibitor part numbers:
- RNase Inhibitor 40x (10x Genomics, PN-1000887)
- Protector RNase Inhibitor (Sigma-Aldrich/MilliporeSigma, PN-3335402001/3335399001)
Third-party Items:
-
See relevant, compatible Demonstrated Protocols/Sample Prep User Guide and Protocol Planner for a comprehensive list
- See the Supported Thermal Cyclers page for thermal cyclers we recommend using with this assay
- See the Sequencing Requirements for Epi Multiome page for supported sequencers and sequencing configurations
-
Before getting started, check out these training tools:
- See the GEM-X Chip Loading Video and GEM-X Training Kit