This document provides best practices for preparing and handling FF tissue samples and generating tissue slides. Protocol outlines fixation, H&E staining, & imaging.

Key companion documents for use with this document:

1. Visium HD 3' Spatial Gene Expression Protocol Planner (CG000803)
2. Tissue Slide Alignment Instruction, Quick Reference Cards (CG000648)
3. Visium S3 for Tissue Slide Cassette Assembly Quick Reference Cards (CG000730)

For use with:

• Visium HD 3' Reagents - Kit A Small, PN-1000854
• Visium HD 3' Reagents - Kit B Small, PN-1000855
• Visium Slide Cassettes S3 6.5 mm, 2 pk, PN-1000847
• Visium HD Slide, 6.5 mm, 2 rxns, PN-1000670
• Visium CytAssist Reagent Accessory Kit, PN-1000499
• Visium CytAssist Alignment Aid, 6.5 mm, PN-1000866