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Quick reference cards for the system component, layout, and software for the Chromium Connect.

Chromium Connect Quick Reference Cards

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Library Construction

Chromium Single Cell Immune Profiling allows analysis of full-length, paired B-cell or T-cell receptor (BCR/TCR) sequences, surface protein expression, antigen specificity, and 5’ gene expression, all from a single cell. Scale up with a high-throughput solution, or focus on key genes with targeted gene expression panels.

Plan ahead by referring to the list of equipment, kits, and reagents needed for Single Cell Immune Profiling.

Experimental Design

Experimental Design & Planning

Prepare optimal single cell/nuclei suspension by following our Demonstrated Protocols.

Sample Prep

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Use pre-designed or custom panels for targeted gene expression, followed by sequencing and data analysis.

Targeted

Targeted Gene Expression (Optional)

Sequence your single cell library as per our provided recommendations.

Sequencing

Analyze your single cell data using our pipelines, visualization, and Cloud Analysis tools.

Software Analysis

Single Cell Immune Profiling

Overview

Documentation

Datasets

Videos

What is Single Cell Immune Profiling?

Measure single cell gene expression, cell surface protein expression, and optionally antigen specificity simultaneously.

Cell Surface Protein (Antigen Specificity optional)

Assess the effects of CRISPR perturbations alongside single cell gene expression.

CRISPR Screening

Enables molecular profiling of 2,000–20,000 cells/sample with simultaneous detection of full length, paired immune receptor sequences, gene and protein expression, and antigen specificity.

High Throughput (HT)

Additional Applications

We provide resources from protocols, user guides, and free-to-use analysis tools.

Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

Chromium Next GEM Single Cell 5' v2 Dual Index

Chromium Next GEM Single Cell 5' HT v2 Dual Index

Chromium Next GEM Single Cell 5' v1.1 with Feature Barcoding

Find answers to common technical questions, from sample prep through data analysis, about Chromium single cell workflows.

Chromium Connect Quick Reference Cards

User Guide, CG000256

CG000256_ChromiumConnect_QuickReferenceCards_RevC.pdf