Videos

Single Cell Gene Expression HT Protocol v3.1 | Break GEMs with Recovery Agent

After the GEMs are transferred into tube strips, you will break GEMs with recovery agent. This video provides an overview of the steps for breaking GEMs including, addition of recovery agent, identification of proper biphasic separation, troubleshooting for incomplete biphasic separation, and removal of recovery agent and partitioning oil. Next step: Post GEM-RT Dynabeads Cleanup.

After the GEMs are transferred into tube strips, you will break GEMs with recovery agent. This video provides an overview of the steps for breaking GEMs including, addition of recovery agent, identification of proper biphasic separation, troubleshooting for incomplete biphasic separation, and removal of recovery agent and partitioning oil. Next step: Post GEM-RT Dynabeads Cleanup.