Videos
Single Cell Gene Expression Protocol v3.1 | Break GEMs with Recovery Agent
After the GEMs are transferred into tube strips, you will break GEMs with recovery agent. This video provides an overview of the steps for breaking GEMs including, addition of recovery agent, identification of proper biphasic separation, troubleshooting for incomplete biphasic separation, and removal of recovery agent and partitioning oil. Next step: Post GEM-RT Dynabeads Cleanup.
Category
How-To Video
Product
Universal 3'
Duration
1 min
Date
Jul 31, 2020