Videos

Single Cell Gene Expression Protocol v3.1 | Break GEMs with Recovery Agent

 

After the GEMs are transferred into tube strips, you will break GEMs with recovery agent. This video provides an overview of the steps for breaking GEMs including, addition of recovery agent, identification of proper biphasic separation, troubleshooting for incomplete biphasic separation, and removal of recovery agent and partitioning oil. Next step: Post GEM-RT Dynabeads Cleanup.

Category

How-To Video

Product

Universal 3'

Duration

1 min

Date

Jul 31, 2020