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4k PBMCs from a Healthy Donor

Universal 3' dataset analyzed using Cell Ranger 1.2.0

Number of samples on this datasets page1

Peripheral blood mononuclear cells (PBMCs) from a healthy donor (same donor as pbmc8k). PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).

  • 4,342 cells detected
  • Sequenced on Illumina Hiseq4000 with approximately 87,000 reads per cell
  • 26bp read1 (16bp Chromium barcode and 10bp UMI), 98bp read2 (transcript), and 8bp I7 sample barcode
  • Analysis run with --cells=5000

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Software

Cell Ranger

Subpipeline

cellranger count

10x Instrument(s)

Chromium Controller

Species

Human

Anatomical Entity

Blood

Disease State

healthy

Cells or Nuclei

Cells

Selected Cell Types

PBMCs

Biomaterial Type

Cell Suspension

Cell/Nuclei Count

4,342

Donor Count

1

Date Published

2017-02-09