500 1:1 Mixture of Fresh Frozen Human (GM12878) and Mouse (A20) Cells (v1.0)

Single Cell ATAC dataset analyzed using Cell Ranger ATAC 1.2.0

Assess data quality

View summary metrics to assess data quality and more.

Visualize and explore data

Discover differentially expressed genes, visualize your favorite genes, and explore your data with our visualization software.

Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

1:1 mixture of human (GM12878) and mouse (A20) cells. This is a classic human-mouse mixture experiment to demonstrate single cell behavior.

Libraries were prepared following the Chromium Single Cell ATAC Reagent Kits (User Guide CG000168 Rev B). All GM12878:A20 mixture libraries (v1.1 chemistry vs. v1 chemistry at all cell loads) were prepared with the same nuclei preparation.

~750 transposed nuclei were loaded. ~500 expected nuclei recovered. 219 human and 233 mouse nuclei were detected.
Sequenced on Illumina NovaSeq with approximately 26k read pairs per cell
- 50 bp Read 1
- 8 bp i7 index (sample index)
- 16 bp i5 (10x Barcode)
- 49 bp Read 2

This dataset is licensed under the Creative Commons Attribution license.