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500 1:1 Mixture of Human HEK293T and Mouse NIH3T3 cells, 3' LT v3.1, Chromium Controller

Universal 3' dataset analyzed using Cell Ranger 6.1.0

Number of samples on this datasets page1

1:1 mixture of fresh frozen human HEK293T and mouse NIH3T3 cells obtained by 10x Genomics from ATCC were used in a classic experiment to demonstrate single cell behavior.

Libraries were generated from ~1630 cells (~500 cells recovered) as described in the Chromium Next GEM Single Cell 3' LT Reagent Kits (v3.1 Dual Index) User Guide (CG000399 RevB) using the Chromium Controller and sequenced on an Illumina NovaSeq 6000 to a read depth of 50k mean reads per cell.

Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript).

Run with --expect-cells=500.

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Additional Applications

Low Throughput

Software

Cell Ranger

Subpipeline

cellranger count

10x Instrument(s)

Chromium Controller

Species

Human, Mouse

Anatomical Entity

Embryonic Kidney, Embryo

Preservation Method

Fresh Frozen

Cells or Nuclei

Cells

Selected Cell Types

fibroblast

Biomaterial Type

Cell Line

Cell/Nuclei Count

611

Donor Count

2

Date Published

2021-08-09