Cryopreserved human peripheral blood mononuclear cells (PBMCs) from a healthy female donor aged 25 were obtained by 10x Genomics from AllCells.
Granulocytes were removed by cell sorting, followed by nuclei isolation as described in the Demonstrated Protocol- Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing (CG000365 Rev A).
Paired ATAC and Gene Expression libraries were generated from the isolated nuclei as described in the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression User Guide (CG000338 Rev A) and sequenced on Illumina Novaseq 6000 v1 Kit (Forward Strand Dual-Index Workflow).
User Guide: Chromium Next Gem Single Cell Multiome ATAC + Gene Expression User Guide (CG000338 Rev A)
Libraries generated:
- Chromium Single Cell Multiome ATAC library
- Chromium Single Cell Multiome Gene Expression library
Sequencing information:
- Sequencer: Novaseq 6000 v1 Kit (Forward Strand Dual-Index Workflow)
- Cycle numbers:
- Gene Expression library: 28,10,10,90
- ATAC library: 50,8,16,49
Key metrics:
- Estimated number of cells: 2,711
- ATAC Mean raw read pairs per cell: 28,482
- ATAC Median high-quality fragments per cell: 14,479
- ATAC Number of peaks: 98,319
- GEX Mean raw reads per cell: 60,701
- GEX Median genes per cell: 1,791
- GEX Median UMI counts per cell: 3,790
- Linked genes: 8,664
- Linked peaks: 53,127