Unsorted PBMCs from a CMV Seropositive Donor - 5' Gene Expression with a Panel of dCODE™ Dextramer® Reagents and TotalSeq™-C Antibodies

Universal 5' dataset analyzed using Cell Ranger 3.0.2

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Dataset overview

Output and supplemental files

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Peripheral blood mononuclear cells (PBMCs) from a CMV seropositive donor, were obtained through a collaboration. Cells were stained with a panel of 12 dCODE™ Dextramer® reagents (Immudex) and 17 TotalSeq™-C antibodies (BioLegend). See details in the Application Note “Redefining Cellular Phenotyping: Comprehensive Characterization and Resolution of the Antigen-Specific T Cell Response”.

Cells were 81% viable by Trypan blue stain.

The same cDNA was used to generate TCR (vdj_v1_hs_unsorted_cmvpos_t) specific libraries.

Libraries were prepared following the Single Cell V(D)J Reagent Kits User Guide (CG000186 RevA).

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.