Immunology

Redefine Your
Understanding of Innate
and Adaptive Immunity

Uncover the Layers
of Immune Cell Complexity

Addressing the intricacies of the immune system leads to actionable insight into infectious disease, autoimmune disorders, immunotherapy development, and cancer. Learn how you can explore immunology with unprecedented breadth, depth, and precision to truly change the course of human health using 10x Genomics technology.

 
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Critical Tools for Coronavirus (COVID-19) Research

The ongoing COVID-19 outbreak has taken thousands of lives and the number of infections is growing daily. With single cell and spatial technology, you can capture multiple dimensions of infection and the immune response. Explore a curated list of COVID-19 publications and other helpful resources.

COVID-19 Resources

Immunology Research Guide

To develop an extensive understanding of the orchestrated innate and adaptive immune response, researchers need tools that can profile tissues at cellular and molecular resolution. Single cell sequencing technologies can be used in exactly this way — to identify the molecular mechanisms of the immune response to pathogens, trace the cell types and states involved, and uncover novel biomarkers for immunotherapy.

Download our comprehensive Immunology Research Guide and learn more about applying single cell sequencing to address the biggest topics in immunology.

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Immunology Experts Take Us Towards a Complete
Understanding of Immunity

Uncover the mechanisms underlying the adaptive and innate immune response, to truly change the course of human health. Learn from fellow immunologists and 10x Genomics scientists about the insights and discoveries now possible with the aid of single cell solutions in these immunology-focused webinars and seminars.

 
High-Throughput B Cell Screening for Immune Profiling and Antibody Discovery in HIV

Speaker: Ian Setliff, laboratory of Ivelin Georgiev, Vanderbilt University

Learn how researchers from Vanderbilt University used single cell immune sequencing to identify a new panel of broadly neutralizing antibodies against HIV-infected B cells.

 
Constructing the Human Immune Census

Speaker: Jimmie Ye, UCSF

Listen to UCSF Assistant Professor Jimmie Ye describe his team’s efforts to build a molecular survey of gene expression in one million immune cells from 200 different blood samples, including samples from patients with lupus.

 
Redefining Immune Cell Phenotyping & Characterization

Speaker: Sarah Taylor, Daniel Riorden, Dagmar Walter

Discover the power of multiomic approaches for phenotyping immune cells as 10x Genomics scientists explain how they used the Chromium Single Cell Immune Profiling Solution to characterize gene expression, T- and B-cell clonotypes and cell surface markers in PBMC samples from a cytomegalovirus (CMV) seropositive and seronegative donor.

 
A New Way of Exploring Immunity

Speaker: Mike Stubbington, Alvaro Martinez Barrio, Dagmar Walter

Explore the capabilities of single cell immune sequencing solutions to characterize the adaptive immune system as 10x Genomics scientists explain how to uncover gene and cell surface protein expression, TCR-pMHC binding specificity, and paired alpha/beta T-cell receptor (TCR) sequences in 160,914 single CD8+ T cells, including 62,858 antigen binding cells.

The Customer Research Snapshot condenses 10x Genomics customer publications into easy-to-understand experimental summaries, detailing how they performed their experiments, analyzed their data and reached their conclusions. Take a look at our growing list of snapshots, showcasing how immunologists are using 10x Genomics products to understand the diverse cellular and molecular mechanisms underlying the immune response.

The Immune Cell Landscape in Kidneys of Patients With Lupus Nephritis

Arazi et al., Nature Immunology 2019

Performed single cell analysis of immune cells in human kidneys affected by lupus nephritis.

High-Throughput Mapping of B Cell Receptor

Setliff et al., Cell 2019

Developed LIBRA-seq, a method to rapidly map paired heavy- and light-chain B cell receptor (BCR) sequences to their corresponding antigens

Gene Expression Variability Across Cells and Species Shapes Innate Immunity

Hagai et al., Nature 2018

Used single cell and bulk RNA sequencing to chart the evolution of the innate immune response in mammals, identifying genes that were differentially expressed upon immune stimulation.

Single Cell Analysis of Fate-mapped Macrophages Reveals Heterogeneity During Atherosclerosis Progression and Regression

Lin et al., JCI Insight 2019

Used single cell RNA sequencing to characterize the cellular states of macrophages in atherosclerotic plaques, showing a spectrum of macrophage activation states.

Capture a Holistic Snapshot of the Immune System

Perform complex phenotyping in tens of thousands of immune cells by incorporating 10x Genomics technology into your existing flow cytometry workflow. See how we did it with these helpful resources.

See how we recently demonstrated the immense scalability of our Feature Barcoding technology to produce a rich dataset elucidating the mechanisms of TCR-pMHC interactions.

Learn about how to perform multiomic phenotyping in single T cells, including cell surface protein and antigen specificity characterization.

Get more in-depth information about how to identify cell-to-cell variation in regulatory elements to better characterize immune cell types.

 

Watch this presentation from AGBT 2019 to see how multiomic approaches can be leveraged to gain a deeper understanding of adaptive immunity.

Getting to the Next Level of Characterization & Resolution

Immunologists around the world are leveraging 10x Genomics products to advance our knowledge of the heterogeneity and developmental progression of immune cells. See how Immunologists are taking their research to the next level by viewing our featured publications below and downloading our Immunology Research Highlights flyer

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High-Throughput Mapping of B Cell Receptor Sequences to Antigen Specificity
I Setliff et al. Cell. 2019
Performed high-throughput interrogation of antibody-antigen interactions using single cell sequencing to screen B cells from human HIV-infection samples against large libraries of viral antigens for antibody discovery.
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Massively parallel single-cell chromatin landscapes of human immune cell development and intratumoral T cell exhaustion
Satpathy AT et al. Nat Biotechnol. 2019
Analyzed serial tumor biopsies before and after PD-1 blockade, revealing chromatin regulators of therapy-responsive T cell subsets and identified a shared regulatory program that governs intratumoral CD8+ T cell exhaustion and CD4+ T follicular helper cell development.
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The bone marrow microenvironment at single-cell resolution
Tikhonova AN et al. Nature. 2019
Mapped the transcriptional landscape of mouse bone marrow microenvironment both at homeostasis and under conditions of stress-induced haematopoiesis revealing previously unappreciated levels of cellular heterogeneity and considerable transcriptional remodelling under stress conditions
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Memory CD4+ T cells are generated in the human fetal intestine
Li N et al. Nat Immunol. 2019
Characterize the CD4+ T cell compartment in the human fetal intestine and found evidence for the generation of memory-like CD4+ T cells in the human fetal intestine that is consistent with exposure to foreign antigens.

Learn More About Our Products to Explore the Immune System With Unprecedented Depth, Breadth, and Precision

The Chromium Single Cell Immune Profiling Solution allows you to simultaneously examine the cellular context of the adaptive immune response and immune repertoires of hundreds to tens of thousands of T and B cells in human or mouse on a cell-by-cell basis.

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The Chromium Single Cell ATAC Solution allows you to discover cellular heterogeneity stemming from epigenetic variability and better understand the regulation of genes to better define cell state and types for lineage tracing and biomarker discovery.

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The Chromium Single Cell Gene Expression Solution provides an unparalleled approach to uncover cell-to-cell gene expression variability and identify rare cell types from complex biological samples.

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The Visium Spatial Gene Expression solution is a next-generation molecular imaging technology that allows you to classify tissue globally based on the total mRNA, or focus on any region of interest to discover novel gene expression profiles.

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Redefine Your Understanding
of Innate and Adaptive Immunity
with Solutions from 10x Genomics