10k 1:1 Mixture of Raji and Jurkat Cells Multiplexed, 2 CMOs

Jurkat and Raji cell lines obtained by 10x Genomics from ATCC were multiplexed at equal proportions with 1 CMO per cell line, resulting in a pooled sample labeled with 2 CMOs.

Libraries were prepared following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Multiplexing User Guide (CG000388) and sequenced on Illumina NovaSeq 6000.

Single Cell 3’ Cell Multiplexing v3.1 Dual Index Library

• Sequencing Depth: 28,266 read pairs per cell
• Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI); i5 index: 10 cycles (sample index); i7 index: 10 cycles (sample index); Read 2: 90 cycles (transcript)

Pooled Multiplexed Sample - Key Metrics

• Estimated Number of Cells: 13,615
• Cells Assigned to a Sample: 11,070

Individual Multiplexed Samples - Key Metrics