All datasets

10k Cells from a MALT Tumor - Gene Expression with a Panel of TotalSeq™-B Antibodies

Universal 3' dataset analyzed using Cell Ranger 3.0.0

Number of samples on this datasets page1

Cells from a dissociated Extranodal Marginal Zone B-Cell Tumor (MALT: Mucosa-Associated Lymphoid Tissue) stained with a panel of TotalSeq™-B antibodies.

  • 8,412 cells detected
  • Sequenced on Illumina NovaSeq with approximately 32,000 reads per cell
  • 28bp read1 (16bp Chromium barcode and 12bp UMI), 91bp read2 (transcript), and 8bp I7 sample barcode
  • run with --expect-cells=10000
  • This dataset was generated using a pre-release set of TotalSeq-B antibodies. The barcode sequences have since changed. Please refer to https://www.biolegend.com/totalseq for the latest conjugated barcode information.

To maintain donor anonymity, sequence data for this sample is not currently available.

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Additional Applications

Cell Surface Protein

Software

Cell Ranger

Subpipeline

cellranger count

10x Instrument(s)

Chromium Controller

Species

Human

Anatomical Entity

Blood

Disease State

B-Cell Tumor (MALT)

Cells or Nuclei

Cells

Selected Cell Types

B-lymphocyte

Biomaterial Type

Specimen from Organism

Cell/Nuclei Count

8,412

Donor Count

1

Date Published

2018-11-19