Pan T cells isolated from mononuclear cells of a healthy donor (same donor as t_4k). T cells are primary cells with relatively small amounts of RNA (~1pg RNA/cell).
- 3,549 cells detected
- Sequenced on Illumina Hiseq4000 with approximately 100,000 reads per cell
- 26bp read1 (16bp Chromium barcode and 10bp UMI), 98bp read2 (transcript), and 8bp I7 sample barcode
- Analysis run with --cells=6000