5 µm section from serous papillary carcinoma of human ovarian. FFPE tissue purchased from iSpecimen.
- Block 108906
- Ovarian Cancer, Serous papillary carcinoma
- Brightfield camera, Magnification: 20x
- Sequencing instrument: Illumina NovaSeq 6000, flow cell HCCVHDSX2 (lanes 1-4)
- Sequencing depth: 63,836 reads per spot
- Sequencing configuration: 28 bp read 1 (16 bp Visium spatial barcode, 12 bp UMI), 75 bp read 2 (transcript), 10 bp i7 sample barcode and 10 bp i5 sample barcode
- Dual-Index set: SI-TS-H1
- Slide: V10L13-020
- Area: D1
Key metrics were:
- Spots detected under tissue: 3,455
- Median genes per spot: 7,621
- Median UMI counts per spot: 28,927