Universal 5' dataset analyzed using Cell Ranger 3.0.0
Peripheral blood mononuclear cells (PBMCs) of a healthy donor, purchased from AllCells (Catalog #: PB001), 87% viable by Trypan blue stain. PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).
The same cDNA was used to generate TCR (vdj_v1_hs_pbmc2_t) and B cell Ig (vdj_v1_hs_pbmc2_b) specific libraries.
Libraries were prepared following the Single Cell V(D)J Reagent Kits User Guide (CG000186 RevA).
This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.
Product
Universal 5'
Additional Applications
Cell Surface Protein
Software
Cell Ranger
Subpipeline
cellranger count
10x Instrument(s)
Chromium Controller
Species
Human
Anatomical Entity
Blood
Disease State
healthy
Cells or Nuclei
Cells
Selected Cell Types
PBMCs
Biomaterial Type
Cell Suspension
Cell/Nuclei Count
8,258
Donor Count
1
Date Published
2018-11-19
