Donor Information: Healthy female.
Isolation protocol: CG000392.RevA: Isolation of Leukocytes, Bone Marrow and Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing.
Whole transcriptome/Gene Expression libraries were generated as described in the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index) User Guide (CG000204 Rev D).
Sequencing data were processed using Cell Ranger, with web_summary.html and output files available in relevant tabs.
- Sequencing instrument: Illumina NovaSeq 6000
- Sequencing depth: 25,226
- Sequencing configuration: Paired-end (28 X 90), single-indexed. Read 1: 28 cycles (16 bp barcode, 10 bp UMI); i7 index: 10 cycles; i5 index: 10 cycles; Read 2: 90 cycles (transcript).
- Cells detected: 4,351
- Median genes per cell: 1,533
- Median UMIs per cell: 4,812