This FF Tissue Handbook provides guidance on:

- Freezing and embedding tissue samples prior to cryosectioning
- Best practices for handling tissue samples and slides before and after cryosectioning
- Hematoxylin and Eosin (H&E) staining to check tissue quality
- Cryosectioning of tissue samples and section placement
- Fixation and pretreatment of tissue sections

Tissue Preparation Handbook is compatible with all Atera assay applications.


Provides step-by-step guidance for preparing fresh frozen (FF) tissues for downstream Atera workflows.

Atera

A high-throughput single-cell spatial imaging platform with automated in situ hybridization, imaging, and on-instrument decoding.

Atera Gene Expression - Fresh Frozen Tissue Preparation Handbook

For proper tissue handling and preparation techniques to preserve the morphology, quality, and integrity of tissue sections, refer to our Demonstrated Protocols for FFPE and Fresh Frozen.

Tissue Prep

The Atera platform sets a new standard for spatial biology by enabling you to analyze the entire transcriptome at unprecedented scale and subcellular resolution, helping to accelerate our understanding of tissue and disease and enable groundbreaking advancements in human health.

Find resources from protocols to free-to-use analysis tools.

Plan ahead by referring to the list of equipment, kits, and reagents needed for Atera.

Experimental Design

Experimental Design & Planning

Learn about the pre-designed Gene Expression panels and engage with 10x design experts to design, order, and manufacture Atera Custom Genes.

Panel Design

Prepare tissue sections for on-instrument analysis by following our step-by-step User Guide/Protocols.

Assay

Refer to instrument-specific documents for seamless instrument operation.

Instrument

Analyze your Atera in situ data using our pipelines and visualization tools.

Software Analysis

Safety Data Sheets

Workflow Documentation

User guides

Knowledge base Q&A

Find answers to common technical questions, from sample prep through data analysis, about Atera workflows.

Access free, publicly available data from a number of different sample types generated with Atera.

Datasets

Other resources

Overview

User Guides

Workflow Overview

Documentation overview and required third-party equipment and reagents for Atera Gene Expression.

Atera Gene Expression Protocol Planner

Step-by-step instructions and visual guides for using the Atera Tissue Placement Aid on OTS slides.

Atera Quick Reference Cards for Tissue Placement on OTS Slides

Provides step-by-step guidance for preparing formalin fixed & paraffin embedded (FFPE) tissues for downstream Atera workflows.

Atera Gene Expression FFPE Tissue Preparation Handbook

Provides step-by-step guidance and visual aids for cassette assembly.

Atera Quick Reference Cards for Slide Cassette Assembly

Tissue Preparation

This document details the protocol for generating Atera Whole Transcriptome (WTA) and Targeted Select Gene Expression data.

Atera Gene Expression User Guide

Comprehensive operating instructions and maintenance guidelines for the Atera Instrument.

Atera Instrument

Step-by-step guidance for post-Atera H&E staining.

Post-Atera H&E

Post-Instrument Applications

Atera Instrument Software enables run set up, Atera Onboard Analysis data generation, and data egress.

Atera Onboard Analysis

Explore in situ data from the tissue scale down to subcellular resolution with our interactive visualization tool.

Atera Gene Expression - Fresh Frozen Tissue Preparation Handbook

Demonstrated Protocol, CG001670

CG001670_AteraFFHandbook_RevA.pdf