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The Chromium X Series Firmware v2.5 update started release on the week of October 27, 2025. This update is being pushed to instruments in phases. Over the weeks following October 27, 2025, all users will receive the update. You will be notified on your connected instrument once the update is available for your device.

To download the latest firmware, navigate to "System" on your instrument and select "Firmware Update" for installation. For help connecting your instrument, refer to our [support site](/support/instruments/chromium-x-series/chromium-x-series-network-connectivity-guidelines) or contact support@10xgenomics.com.

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FOR USE WITH:

- Chromium X & Accessory Kit, 12-Month Warranty, PN-1000331
- Chromium iX Accessory Kit, PN-1000323
- Chromium X Upgrade Kit, PN-1000327
- Chromium X & Accessory Kit, 24-Month Warranty, PN-1000332
- Chromium iX Accessory Kit, PN-1000323
- Chromium X Upgrade Kit, PN-1000327
- Chromium iX & Accessory Kit, 12-Month Warranty, PN-1000328
- Chromium iX Accessory Kit, PN-1000323
- Chromium iX & Accessory Kit, 24-Month Warranty, PN-1000329
- Chromium iX Accessory Kit, PN-1000323

<LinkableSection title={"v2.5.0 (October, 2025)"}>

#### Features

- Added a Remote Support feature for use when prompted by 10x Support teams.
- Enabled connection to WPA-EAP TLS WiFi networks.
- Incorporated security fixes in instrument firmware.

</LinkableSection>

<LinkableSection title={"v2.4.0 (May, 2025)"}>

#### Features

- Updated connectivity status icons shown in header bar for improved clarity.
- The currently connected WiFi network is now shown at the top of the list of available networks.
- The ethernet settings menu can now be accessed without a cable plugged into the instrument.

#### Bug Fixes

- Fixed a bug where an inserted USB was sometimes not selectable in UI.
- Improved error handling to prevent runs from starting when the instrument is in an unhealthy state.

</LinkableSection>

<LinkableSection title={"v2.3.0 (February, 2025)"}>

#### Features

- Added a network diagnostic test to assist with connectivity troubleshooting
- Added a new warning message that alerts users when the instrument experiences a system problem while idle

#### Bug Fixes

- Clarified error messages for incompatible and unreadable chips
- Improved stability of ongoing firmware updates when a newer update becomes available during the installation process
- Made other improvements to logging for enhanced supportability

</LinkableSection>

<LinkableSection title={"v2.2.0 (September, 2024)"}>

#### Features

- Added support for the new instrument in the Chromium X Series: Chromium Xo

</LinkableSection>

<LinkableSection title={"v2.1.0 (July, 2024)"}>

#### Features

- The current firmware version is now displayed in the main screen header bar for easier firmware status checking
- Clarified error message for chip compatibility when a firmware update is required
- Improved support for enterprise WiFi authentication schemes (WPA2 MSCHAPv2)
- Improved connectivity settings to support static IPs

#### Bug Fixes

- Improved the stability of the firmware upgrade process. The instrument will automatically reboot if there is insufficient memory to complete the upgrade
- Reduced the incidence of falsely reported failed Readiness Tests
- Reduced the incidence of tray motion errors
- Other improvements and clarifications to logging and error reporting

</LinkableSection>

<LinkableSection title={"v2.0.0 (March, 2024)"}>

#### Features

- Added support for GEM-X chips
- Added Fluidics Flush Alternative Mode for use when prompted by 10x Genomics Technical Support
- Improved connectivity settings
- Improved interface for performing a soft power down

#### Bug Fixes

- Fixed progress bar behavior during log uploads
- Display permanent WiFi MAC address in Network Settings

</LinkableSection>

<LinkableSection title={"v1.2.2 (June, 2023)"}>

#### Bug Fixes

- Improved clamp pressure to reduce chances of unintentional liquid release during a run
- Eliminated unnecessary delay at run completion

</LinkableSection>

<LinkableSection title={"v1.2.1 (April, 2023))"}>

#### Features

- Enhanced diagnostic logging to improve troubleshooting
- Improved monitoring of hardware components

#### Bug Fixes

- Improved integration with third-party clock battery firmware, reducing the risk of impact to run performance
- Fixed bug leading to false positive EC-0074 errors
- Fixed bug resulting in run index incrementing by more than one
- Fixed bug preventing run logs from being visible on the instrument user interface
- Fixed bug that occasionally prevented the tray from closing
- Improved scrolling behavior reduces the likelihood of accidental clicks

</LinkableSection>

<LinkableSection title={"v1.2.0 (February, 2023)"}>

#### Features

- Enhanced diagnostic logging to improve troubleshooting
- Improved monitoring of hardware components

#### Bug Fixes

- Improved integration with third party clock battery firmware, reducing risk of impact to run performance
- Fixed bug leading to false positive EC-0074 errors
- Fixed bug resulting in run index incrementing by more than one
- Improved scrolling behavior reducing the likelihood of accidental clicks

</LinkableSection>

<LinkableSection title={"v1.1.0 (March, 2022)"}>

#### Features

- New assay compatibility- Fixed RNA Profiling
- Enhanced error detection and troubleshooting capabilities
- Ethernet and Wi-Fi MAC addresses are accessible
- The instrument’s serial number is set as the hostname enabling all Chromium X Series instruments to have a unique hostname

#### Bug Fixes

- Fixed Wi-Fi icon display in the connection status
- Disabled bypassing critical components installation during firmware update

</LinkableSection>


Notification of new features for Chromium X Series firmware

Chromium X Series

Chromium

Gene Expression

Epigenomic Profiling

Immune Profiling

Protein co-detection

Advanced system for multiomic analysis of hundreds to millions of cells.

Error

GEM-X technology-powered assays with whole transcriptome coverage to gather broad information from diverse species

Universal 3'

3' gene expression profiling at scale with single cell resolution.

GEM-X technology-powered assays with whole transcriptome coverage to analyze immune diversity 

Universal 5'

5' gene expression alongside V(D)J repertoire profiling and antigen specificity of T and B cells.

Plate-based sample handling brings unprecedented scale to highly sensitive single cell profiling

Flex

Fixed RNA Profiling assay for comprehensive probe-based gene expression profiling with single cell resolution.

Epi ATAC

Chromatin accessibilility profiling at the single cell level.

Single cell profiling of open chromatin and 3' gene expression from the same nucleus

Epi Multiome ATAC + Gene Expression

Combined profiling of 3' gene expression and chromatin accessibility from the same cell.

Chromium X Series Firmware Release Note

Chromium Epi Multiome ATAC + Gene Expression performs direct measurements of 3' gene expression and chromatin accessibility from the same cell.

Find resources from protocols to free-to-use analysis tools.

Plan ahead by referring to the list of equipment, kits, and reagents needed for Single Cell Multiome ATAC + Gene Expression.

Experimental Design

Experimental Design & Planning

Prepare optimal single cell/nuclei suspension by following our Demonstrated Protocols.

Sample Prep

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Library Construction

Sequence your single cell library as per our provided recommendations.

Sequencing

Analyze your single cell data using our pipelines, visualization, and Cloud Analysis tools.

Software Analysis

Workflow Documentation

Overview

User Guides

Sample prep

Library prep

Software

Datasets

Videos

Publications

What is Epi Multiome ATAC + Gene Expression?

Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

Chromium Single Cell Multiome ATAC and Gene Expression

Chromium Next GEM Epi Multiome ATAC + Gene Expression

Find answers to common technical questions, from sample prep through data analysis, about Chromium single cell workflows.

Read key peer-reviewed research publications offering greater insights into biology, enabled by 10x Genomics products and technologies.