FOR USE WITH:

GEM-X Epi Multiome

- GEM-X Epi Multiome, 16 samples PN-1000979 | 4 samples PN-1000980
  - GEM-X Epi Multiome GEM Kit, 16 rxns PN-1000945 | 4 rxns PN-1000946
  - GEM-X Epi Multiome Amp Kit, 16 rxns PN-1000947 | 4 rxns PN-1000948
  - GEM-X Epi Multiome Gel Bead Kit 16 rxns PN-1000943 | 4 rxns PN-1000944
  - GEM-X Epi Multiome ATAC Kit 16 rxns PN-1000949 | 4 rxns PN-1000950
  - Library Construction Kit C, 16 rxns PN-1000694 | 4 rxns PN-1000689
  - Dynabeads™ MyOne™ SILANE
- GEM-X Epi Multiome Chip Kit, 4 chips, PN-1000951
- Single Index Kit N Set A, 96 rxns, PN-1000212
- Dual Index Kit TT Set A, 96 rxns, PN-1000215


Provides workflow overviews, document resources, reagents, and consumables for the GEM-X Epi Multiome protocol.

Single cell profiling of open chromatin and 3' gene expression from the same nucleus

Epi Multiome

Chromium X Series

Chromium

Advanced system for multiomic analysis of hundreds to millions of cells.

Gene Expression

Epigenomic Profiling

Immune Profiling

Protein co-detection

Combined profiling of 3' gene expression and chromatin accessibility from the same nucleus.

GEM-X Epi Multiome Protocol Planner

Plan ahead by referring to the list of equipment, kits, and reagents needed for Epi Multiome.

Experimental Design

Experimental Design & Planning

Chromium Epi Multiome performs direct measurements of 3' gene expression and chromatin accessibility from the same nucleus.

We provide resources from Demonstrated Protocols, User Guides, and free-to-use analysis tools.

Prepare optimal single cell/nuclei suspensions by following our Demonstrated Protocols or Sample Prep User Guide.

Sample Prep

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol from nucleus samples.

Library Construction

Sequence your single nucleus library as per our provided recommendations.

Sequencing

Analyze your single nuclei data using our pipelines, visualization, and Cloud Analysis tools.

Software Analysis

Workflow Documentation

Overview

User Guides

Sample prep

Library prep

Software

Datasets

Videos

Publications

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What is Epi Multiome?

Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

GEM-X: How to assemble, load and generate GEMs

Chromium Single Cell Multiome ATAC and Gene Expression

Chromium Next GEM Epi Multiome

Find answers to common technical questions, from sample prep through data analysis, about Chromium single cell workflows.

Read key peer-reviewed research publications offering greater insights into biology, enabled by 10x Genomics products and technologies.

GEM-X Epi Multiome Protocol Planner

User Guide, CG001688

CG001688_GEM-X_Epi_Multiome_Planner_RevA.xlsm