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Nuclei Isolation for Single Cell ATAC Sequencing

  • CG000169_DemonstratedProtocol_NucleiIsolation_ATAC_Sequencing_Rev E.pdf

    Demonstrated Protocol, CG000169

    CG000169_DemonstratedProtocol_NucleiIsolation_ATAC_Sequencing_Rev E.pdf

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This protocol outlines how to isolate, wash, and count nuclei suspensions for use with the Chromium Single Cell ATAC protocol. Cryopreserved primary cells (PBMCs) and cell lines (GM12878 cells; EL4 cells) were used to develop this protocol. PBMCs were cryopreserved in IMDM + 40% FBS + 15% DMSO. Cell lines were cryopreserved in RPMI + 15% FBS + 5% DMSO. Optimization of some protocol steps (e.g. lysis time, centrifugation speed/time and filtration steps) may be needed based on cell type.

This protocol is only compatible with the Chromium Single Cell ATAC reagent kits. Do not use with Multiome ATAC + Gene Expression or SC3'v3.1 assays.

FOR USE WITH:

  • Chromium Single Cell ATAC Reagent Kits User Guide (v1.1 Chemistry) (CG000209)
  • Chromium Single Cell ATAC Reagent Kits User Guide (v1 Chemistry) (CG000168)
  • Chromium Next GEM Single Cell ATAC Reagent Kits v2 User Guide (CG000496)
Document Type
Demonstrated Protocol

Last Modified
June 17, 2022