The Chromium Single Cell ATAC Solution produces Illumina® sequencer-ready libraries.
Single Cell ATAC Libraries (v1, v1.1, and v2 Chemistries)
 | Sequencing requirements of Multiome ATAC libraries require different considerations, refer to the Sequencing Requirements for Single Cell Multiome ATAC + Gene Expression page for specifications. |
Supported Sequencers:*
- Illumina® NovaSeq
- Illumina® HiSeq 3000/4000
- Illumina® HiSeq 2500
- Illumina® NextSeq 500/550
- Illumina® NextSeq 1000/2000
- Illumina® MiSeq
Recommended Sequencing Depth: 25,000 read pairs per nucleus (50,000 individual reads. 25,000 from R1, 25,000 from R2)
Dual-Indexed Sequencing Run: Single Cell ATAC libraries are dual-indexed.
PhiX Spike-In Recommendations: 1%.
| Read | Read 1 | i7 Index | i5 Index | Read 2 |
|---|---|---|---|---|
| Purpose | Transposed DNA | Sample Index | 10x Barcode | Transposed DNA |
| Length | 50 | 8 | 16 | 50 ** |
* Do not pool Chromium Single Cell ATAC libraries with other 10x libraries while sequencing
** Can be 1 bp shorter to accommodate NovaSeq v1.0 100 cycle kits