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Single Cell Immune Profiling

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What is Single Cell Immune Profiling?

Chromium Single Cell Immune Profiling allows analysis of full-length, paired B-cell or T-cell receptor (BCR/TCR) sequences, surface protein expression, antigen specificity, and 5’ gene expression, all from a single cell. Scale up with a high-throughput solution, or focus on key genes with targeted gene expression panels.

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Documentation

We provide resources from protocols, user guides, and free-to-use analysis tools.

Experimental Design & Planning

Plan ahead by referring to the list of equipment, kits, and reagents needed for Single Cell Immune Profiling.

Sample Prep

Prepare optimal single cell/nuclei suspension by following our Demonstrated Protocols.

Instruments

Refer to instrument-specific documents for seamless instrument operation.

Library Construction

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Targeted Gene Expression (Optional)

Use pre-designed or custom panels for targeted gene expression, followed by sequencing and data analysis.

Sequencing

Sequence your single cell library as per our provided recommendations.

Software Analysis

Analyze your single cell data using our pipelines, visualization, and Cloud Analysis tools.

Additional Applications

Cell Surface Protein (Antigen Specificity optional)
Measure single cell gene expression, cell surface protein expression, and optionally antigen specificity simultaneously.
CRISPR Screening
Assess the effects of CRISPR perturbations alongside single cell gene expression.
High Throughput (HT)
Enables molecular profiling of 2,000–20,000 cells/sample with simultaneous detection of full length, paired immune receptor sequences, gene and protein expression, and antigen specificity.
Datasets

Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

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Datasets

Videos

Chromium Next GEM Single Cell 5' v2 Dual Index video
How-to series
Chromium Next GEM Single Cell 5' v2 Dual Index
Chromium Next GEM Single Cell 5' HT v2 Dual Index video
How-to series
Chromium Next GEM Single Cell 5' HT v2 Dual Index
Chromium Next GEM Single Cell 5' v1.1 with Feature Barcoding video
How-to series
Chromium Next GEM Single Cell 5' v1.1 with Feature Barcoding
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Q&As

Find answers to common technical questions, from sample prep through data analysis, about Chromium single cell workflows.

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Q&As