10x Genomics Support/Space Ranger 2.1/Introduction/

Direct Placement Workflow for Fresh Frozen

The Visium Spatial Gene Expression workflow measures mRNA levels in intact, fresh frozen (FF) tissue sections using spatially barcoded oligonucleotides attached to the Visium slide. The workflow begins by first determining the optimal permeabilization time for the tissue. The tissue is stained, imaged, and cell permeabilized to release mRNA. The mRNA binds to the poly(dT) region of spatially barcoded oligonucleotides present on the slide surface. Libraries are generated from the barcoded cDNA of a captured transcript and are sequenced.

Resources and additional information are available below: