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1k 1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells (Next GEM)

Single Cell Gene Expression dataset analyzed using Cell Ranger 3.0.2

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1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells.

This is a classic human-mouse mixture experiment to demonstrate single cell behavior (the same cells were used to generate 1k_hgmm_v3, 5k_hgmm_v3_nextgem, 10k_hgmm_v3_nextgem)).

Libraries were prepared following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 User Guide (CG000204 RevA).

  • 1,230 cells detected
  • Sequenced on Illumina NovaSeq with approximately 66,606 reads per cell
  • 28bp read1 (16bp Chromium barcode and 12bp UMI), 91bp read2 (transcript), and 8bp I7 sample barcode
  • run with --expect-cells=1000

This dataset is licensed under the Creative Commons Attribution license.