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1k 1:1 Mixture of Human HEK293T and Mouse NIH3T3 Cells, 3’ v3.1

Single Cell Gene Expression dataset analyzed using Cell Ranger 6.0.0

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1:1 mixture of fresh frozen human HEK293T and mouse NIH3T3 cells obtained by 10x Genomics from ATCC were used in a classic experiment to demonstrate single cell behavior.

Libraries were generated following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) User Guide (CG000315) and sequenced on Illumina NovaSeq 6000.

Sequencing Depth: 57,015 mean reads per cell

Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript)

Analysis Parameters Used: Run with --expect-cells=1000

This dataset is licensed under the Creative Commons Attribution license.