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10k 1:1 Mixture of Fresh Frozen Human (GM12878) and Mouse (A20) Cells

Epi ATAC dataset analyzed using Cell Ranger ATAC 1.1.0

Number of samples on this datasets page1

1:1 mixture of human (GM12878) and mouse (A20) cells. This is a classic human-mouse mixture experiment to demonstrate single cell behavior.

  • ~15,500 transposed nuclei were loaded.
  • 5,438 human and 4,902 mouse nuclei were recovered.
  • Sequenced on Illumina NovaSeq with approximately 41k read pairs per cell.
    • 50 bp Read 1
    • 8 bp i7 index (sample index)
    • 16 bp i5 (10x Barcode)
    • 49 bp Read 2

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Epi ATAC

Software

Cell Ranger ATAC

Subpipeline

cellranger-atac count

10x Instrument(s)

Chromium Controller

Species

Human, Mouse

Anatomical Entity

Blood

Preservation Method

Fresh Frozen

Disease State

lymphoma

Cells or Nuclei

Nuclei

Selected Cell Types

B-lymphocyte

Biomaterial Type

Cell Suspension

Cell/Nuclei Count

10,340

Donor Count

2

Date Published

2019-04-16