Mouse peripheral blood mononuclear cells (PBMCs) from a C57BL/6 strain (age 8 weeks, male) were obtained by 10x Genomics from C&M LabPro and multiplexed at equal proportions with 2 CMOs.
The config.csv input file was submitted with two sample IDs (correlating to the two individually tagged aliquots of mouse PBMCs) and with one CMO ID assigned per sample ID.
Libraries were prepared following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Multiplexing User Guide (CG000388) and sequenced on Illumina NovaSeq 6000.
Single Cell 3’ Cell Multiplexing v3.1 Dual Index Library
- Sequencing Depth: 23,666 read pairs per cell
- Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI); i5 index: 10 cycles (sample index); i7 index: 10 cycles (sample index); Read 2: 90 cycles (transcript)
Pooled Multiplexed Sample - Key Metrics
- Estimated Number of Cells: 10,521
- Cells Assigned to a Sample: 9,834
Individual Multiplexed Samples - Key Metrics
| Metric | PBMCs Mouse 1 | PBMCs Mouse 2 |
|---|---|---|
| Cells Assigned to a Sample | 4,598 | 5,236 |
| Median Genes per Cell | 1,412 | 1,414 |
| Median UMI Counts per Cell | 3,936 | 3,929 |