10k Mouse Kidney FFPE Tissue Dissociated using gentleMACS Dissociator, Singleplex Sample
Single Cell Gene Expression Flex dataset analyzed using Cell Ranger 7.1.0
Learn about Chromium analysis
A whole kidney was harvested from an adult mouse (male C57BL/6, 66 days old) and fixed with 10% neutral-buffered formalin overnight at 4°C, and processed in-house into a formalin-fixed paraffin-embedded (FFPE) blocks. Cells were isolated from three, 50 micron FFPE curls (sagittal section) following the demonstrated protocol Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling (CG000632), using the gentleMACS Octo Dissociator option.
The Fixed RNA Gene Expression library was generated as described in the Chromium Fixed RNA Profiling for Singleplexed Samples with Feature Barcode technology for Cell Surface Protein User Guide (CG000477). The resulting library was sequenced on an Illumina NovaSeq6000 with approximately 14k read pairs per cell.
- Total 9,616 cells detected
- Paired-end, dual indexing: 28 cycles Read 1, 10 cycles i7, 10 cycles i5, 90 cycles Read 2
Brightfield and propidium iodide fluorescence images of the resulting cell suspensions after dissociation are provided, as well as images of the final pooled sample (also shown below).
| Brightfield | Fluorescence |
|---|---|
 |  |
This dataset is licensed under the Creative Commons Attribution license.