Peripheral blood mononuclear cells (PBMCs) from two healthy human male donors, aged 18-35 and two healthy female donors, aged 36-50 were obtained by 10x Genomics from Cellular Technologies Limited.
Gene Expression libraries were generated as described in the GEM-X Universal 3' Gene Expression v4 4-plex On-chip Multiplexing User Guide (CG000768) and sequenced on an Illumina NovaSeq 6000 with approximately 53,000 read pairs per cell for the Gene Expression library.
Paired-end, dual indexing libraries were sequenced with this configuration:
- 28 cycles Read 1
- 10 cycles i7
- 10 cycles i5
- 90 cycles Read 2
Libraries were analyzed using the cellranger multi pipeline.
See companion datasets: