All datasets

5k Human PBMCs (Donor 3)

Universal 3' dataset analyzed using Cell Ranger 9.0.0

Number of samples on this datasets page1

Peripheral blood mononuclear cells (PBMCs) from a healthy human female donor, aged 36-50 were obtained by 10x Genomics from Cellular Technologies Limited.

Gene Expression libraries were generated as described in the Chromium GEM-X Single Cell 3' Reagent Kits v4 User Guide (CG000731) and sequenced on an Illumina NovaSeq 6000 with approximately 39,000 read pairs per cell for the Gene Expression library.

Paired-end, dual indexing libraries were sequenced with this configuration:

  • 28 cycles Read 1
  • 10 cycles i7
  • 10 cycles i5
  • 90 cycles Read 2

Libraries were analyzed using the cellranger multi pipeline.

See companion datasets:

  • 5k Human PBMCs (Donor 1)
  • 5k Human PBMCs (Donor 2)
  • 5k Human PBMCs (Donor 4)
  • 20k Human PBMCs (Donor 1-4), Multiplex Sample

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Software

Cell Ranger

Subpipeline

cellranger multi

10x Instrument(s)

Chromium X Series

Species

Human

Anatomical Entity

Blood

Preservation Method

Cryopreserved

Cells or Nuclei

Cells

Selected Cell Types

PBMCs

Biomaterial Type

Cell Suspension

Cell/Nuclei Count

4,773

Donor Count

1

Date Published

2024-11-18