5k Peripheral Blood Mononuclear Cells (PBMCs) from a Healthy Donor (Next GEM)
Single Cell Gene Expression dataset analyzed using Cell Ranger 3.0.2
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
Peripheral blood mononuclear cells (PBMCs) from a healthy donor (the same cells were used to generate 5k_pbmc_v3).
PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).
Libraries were prepared following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 User Guide (CG000204 RevA).
- 5,155 cells detected
- Sequenced on Illumina NovaSeq with approximately 76,406 reads per cell
- 28 bp Read 1 (16 bp Chromium barcode and 12 bp UMI)
- 91 bp Read 2 (transcript)
- 8 bp i7 index (10x Barcode)
- run with
--expect-cells=5000
This dataset is licensed under the Creative Commons Attribution license.