Mouse Splenocytes: 5’v2 Whole Transcriptome Analysis

Single Cell Immune Profiling dataset analyzed using Cell Ranger 6.0.1

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Dataset overview

Output and supplemental files

Input files

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C57Bl/6 mouse splenocytes were obtained by 10x Genomics from C&M Lab Pro. Whole transcriptome/Gene Expression libraries were generated as described in the Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) User Guide (CG000331 Rev A). This whole transcriptome library was used as the parent library for downstream target enrichment. Data were analyzed with Cell Ranger 6.0.1.

Sequencing instrument: Illumina NovaSeq 6000
Sequencing depth: 46,637 mean reads per cell
Sequencing configuration: Paired-end (28 X 90), single-indexed. Read 1: 28 cycles (16 bp barcode, 10 bp UMI); i7 index: 10 cycles; i5 index: 10 cycles; Read 2: 90 cycles (transcript).
Cells detected: 1,358
Median genes per cell: 1,844
Median UMIs per cell: 4,372

This dataset is licensed under the Creative Commons Attribution license.