All datasets

Peripheral blood mononuclear cells (PBMCs) from a healthy donor - Chromium Connect (channel 1)

Universal 3' dataset analyzed using Cell Ranger 3.1.0

Number of samples on this datasets page1

PBMCs isolated and cryopreserved by AllCells. PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).

Libraries were prepared following the Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits v3.1 (CG000286 RevA) and Software version Assay Build 308. 8 libraries were prepared on 1 chip in 1 run. This is channel 1.

  • 5,000 cells targeted
  • 3,996 cells detected
  • Sequenced on Illumina NovaSeq with approximately 70,033 reads per cell
  • 28bp read1 (16bp Chromium barcode and 12bp UMI), 91bp read2 (transcript), and 8bp I7 sample barcode
  • run with --expect-cells=5000

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Software

Cell Ranger

Subpipeline

cellranger count

10x Instrument(s)

Chromium Connect

Species

Human

Anatomical Entity

Blood

Preservation Method

Cryopreserved

Disease State

healthy

Cells or Nuclei

Cells

Selected Cell Types

PBMCs

Biomaterial Type

Cell Suspension

Cell/Nuclei Count

3,996

Donor Count

1

Date Published

2020-02-28