GEM-X technology-powered assays with whole transcriptome coverage to gather broad information from diverse species

Universal 3'

Chromium X Series

Chromium

Advanced system for multiomic analysis of hundreds to millions of cells.

Gene Expression

Epigenomic Profiling

Immune Profiling

Protein co-detection

3' gene expression profiling at scale with single cell resolution.

GEM-X technology-powered assays with whole transcriptome coverage to analyze immune diversity 

Universal 5'

5' gene expression alongside V(D)J repertoire profiling and antigen specificity of T and B cells.

Single cell profiling of open chromatin and 3' gene expression from the same nucleus

Epi Multiome ATAC + Gene Expression

Combined profiling of 3' gene expression and chromatin accessibility from the same cell.

Plate-based sample handling brings unprecedented scale to highly sensitive single cell profiling

Flex

Fixed RNA Profiling assay for comprehensive probe-based gene expression profiling with single cell resolution.

Visium CytAssist

Visium

Compact instrument designed to simplify the Visium workflow by facilitating the transfer of analytes from standard glass slides to Visium slides.

CytAssist Spatial Gene Expression

Probe-based assay allowing simultaneous analysis of molecular and imaging data from FFPE, Fresh Frozen, and Fixed Frozen tissue sections.

HD Spatial Gene Expression

Whole transcriptome probe-based gene expression assay at single cell scale, compatible with FFPE, Fresh Frozen, and Fixed Frozen tissue sections

Combine whole transcriptome spatial analysis with high-plex protein detection using NGS in the same tissue section. Gain a deeper, more holistic understanding of tissue organization, using Visium CytAssist to expand sample access and ease workflows.

Immunofluorescence protein co-detection

Visium CytAssist Gene and Protein Expression

Tissue profiling with transcriptomics and <em>protein co-detection</em>

CytAssist Spatial Gene and Protein Expression

Simultaneous analysis of RNA and protein expression of FFPE tissues, using probe-based chemistry and tagged antibodies.

Spatial Gene Expression for Fresh Frozen

Poly-A based assay allowing simultaneous analysis of molecular and imaging data from fresh frozen tissue sections.

RNase Inhibitor Safety Data Sheets

Construct a sequencing-ready Visium spatial library from your FFPE tissue sections.

Library Construction

CytAssist Spatial Gene Expression analyzes the whole transcriptome of FFPE, Fresh Frozen, or Fixed Frozen tissue sections in a spatially resolved manner

Whole transcriptome analysis of fresh frozen tissue sections utilizing probe-based chemistry.

CytAssist Spatial Gene Expression for Fresh Frozen

Visium CytAssist Instrument facilitates the transfer of analytes from standard glass slides to Visium slides. Find the Instrument Documentation via the button below.

Visium CytAssist Instrument

Visium CytAssist Spatial Gene Expression User Guide

Whole transcriptome analysis of fixed frozen tissue sections utilizing probe-based chemistry.

CytAssist Spatial Gene Expression for Fixed Frozen

Additional Applications

Find resources from protocols to free-to-use analysis tools.

Documentation for Fresh Frozen and Fixed Frozen Applications.

Frozen Tissue Applications

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium CytAssist Spatial Gene Expression protocols.

Experimental Design

Experimental Planning & Design

Best practices for handling FFPE tissue samples and slides before and after sectioning. Guidance for removing hardset coverslips from archived tissue slides.

Tissue Prep

Deparaffinize, stain, image, and decrosslink FFPE tissue sections.

Tissue Staining

For imaging hardware, general image acquisition and analysis guidelines, and example images.

Imaging

Find a list of probes targeting protein-coding genes in human or mouse transcriptome for the Visium CytAssist Spatial Gene Expression assay.

Probe Sets

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Sequence your Visium spatial library as per our provided recommendations.

Sequencing

Analyze your Visium spatial data using our pipelines and visualization tools.

Software Analysis

Workflow Documentation

Overview

User Guides

Datasets

Videos: CytAssist - FFPE

Videos: CytAssist - Frozen

Publications

CytAssist Spatial Gene Expression analyzes the whole transcriptome of FFPE, Fresh Frozen, or Fixed Frozen tissue sections in a spatially resolved manner by mapping gene expression over a high resolution microscope image of the tissue.

What is CytAssist Spatial Gene Expression?

Access free, publicly available data from a number of different sample types generated with Visium Spatial solutions

Videos - CytAssist Spatial Gene Expression

Tissue Preparation Guide

Deparaffinization, H&E Staining, Imaging & Decrosslinking

Find answers to common technical questions, from sample prep through data analysis, about Visium spatial workflows.

Read key peer-reviewed research publications offering greater insights into biology, enabled by 10x Genomics products and technologies.

RNase Inhibitor Safety Data Sheets

SDS,

1000887_RNase_Inhibitor_40x_SDS_Ed1.pdf