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10x Genomics Support/Visium CytAssist Spatial Gene Expression/

Documentation

Find resources from protocols to free-to-use analysis tools.
Frozen Tissue Applications

Documentation for Fresh Frozen and Fixed Frozen Applications.

Experimental Planning & Design

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium CytAssist Spatial Gene Expression protocols.

Tissue Prep

Best practices for handling FFPE tissue samples and slides before and after sectioning. Guidance for removing hardset coverslips from archived tissue slides.

Tissue Staining

Deparaffinize, stain, image, and decrosslink FFPE tissue sections.

Imaging

For imaging hardware, general image acquisition and analysis guidelines, and example images.

Probe Sets

Find a list of probes targeting protein-coding genes in human or mouse transcriptome for the Visium CytAssist Spatial Gene Expression assay.

Library Construction

Construct a sequencing-ready Visium spatial library from your FFPE tissue sections.

Instruments

Refer to instrument-specific documents for seamless instrument operation.

Sequencing

Sequence your Visium spatial library as per our provided recommendations.

Software Analysis

Analyze your Visium spatial data using our pipelines and visualization tools.

Additional Applications