10x
Support
Search Support
Contact us
10x Genomics Support/Visium CytAssist Spatial Gene Expression/Additional Applications/

CytAssist Spatial Gene Expression for Fixed Frozen

What is CytAssist Spatial Gene Expression for Fixed Frozen (FxF)?

Whole transcriptome analysis of mouse fixed frozen OCT embedded tissue sections utilizing probe-based chemistry.

This assay utilizes the following 10x products:

  • Visium CytAssist Spatial Gene Expression for FFPE Reagent Kits
  • Visium CytAssist Instrument and Accessory Kit, to learn more click here

This assay has been validated with the following:

  • Paraformaldehyde (PFA) fixed, sucrose cryopreserved, OCT embedded tissue blocks
  • 10-20 µm section thickness
  • H&E Staining
  • Tissue types (Tested Tissue List)

10x Visium Kit Compatibility Table

Analyte Capture Method10x SolutionFFPE TissueFresh Frozen TissueFixed Frozen Tissue
CytAssist Enabled, Probe-based chemistryVisium CytAssist Spatial Gene Expression
Direct Placement, Probe-based chemistryVisium Spatial Gene Expression for FFPE--
Direct Placement, Poly-A based chemistryVisium Spatial Gene Expression for Fresh Frozen--

Documentation

user-guide

Visium CytAssist Instrument facilitates the transfer of analytes from standard glass slides to Visium slides. Find the Instrument Documentation via the button below.

Find Documentation
    Software
    Q&A
    Videos
    Visium CytAssist Instrument video
    How-to series
    Visium CytAssist Instrument
    Visium CytAssist Spatial Gene Expression User Guide video
    How-to series
    Visium CytAssist Spatial Gene Expression User Guide
    Last Modified
    April 17, 2022