Spatial Gene Expression for Fresh Frozen
What is Spatial Gene Expression for Fresh Frozen?
Visium Spatial Gene Expression measures the whole transcriptome of intact fresh frozen sections in a spatially resolved manner by mapping gene expression over a high-resolution microscope image of the H&E- or IF-stained tissue.
Find resources, from protocols to free-to-use analysis tools.
Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium Spatial protocol.
For proper tissue handling and preparation techniques to preserve the morphology, quality, and integrity of tissue sections, refer to our Tissue Preparation Guide.
Methanol fix, stain, and image tissue following our Demonstrated Protocol.
Optimize permeabilization conditions for your fresh frozen tissue sections by following our step-by step User Guide.
For imaging hardware, general image acquisition and analysis guidelines, and example images, refer to our Technical Note.
Construct a sequencing-ready Visium spatial library from your fresh frozen tissue sections following our step-by-step User Guide.
Use pre-designed panels for targeted gene expression, followed by sequencing and data analysis.
Sequence your Visium spatial library as per our provided recommendations.
Analyze your Visium spatial data using our pipelines and visualization tools.