For imaging hardware, general image acquisition and analysis guidelines, and example images, refer to our Technical Note.

Imaging

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium Spatial protocol.

Experimental Design

Experimental Design & Planning

For proper tissue handling and preparation techniques to preserve the morphology, quality, and integrity of tissue sections, refer to our Tissue Preparation Guide.

Tissue Prep

Methanol fix, stain, and image tissue following our Demonstrated Protocol.

Tissue Staining

Optimize permeabilization conditions for your fresh frozen tissue sections by following our step-by step User Guide.

Tissue Optimization

Construct a sequencing-ready Visium spatial library from your fresh frozen tissue sections following our step-by-step User Guide.

Library Construction

Use pre-designed panels for targeted gene expression, followed by sequencing and data analysis.

Targeted

Targeted Gene Expression (Optional)

<DocumentCallout><Text as={'p'} size={'medium'}>{'After June 30, 2023, new Cell Ranger and Space Ranger releases will no longer support targeted gene expression analysis.'}</Text></DocumentCallout>

Sequence your Visium spatial library as per our provided recommendations.

Sequencing

Analyze your Visium spatial data using our pipelines and visualization tools.